Review



tie2 recombinant protein  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems tie2 recombinant protein
    Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of <t>TIE2</t> stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.
    Tie2 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/pmc12823351-218-39-51
    Average 94 stars, based on 10 article reviews
    tie2 recombinant protein - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes"

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    Journal: Journal of Histochemistry and Cytochemistry

    doi: 10.1369/00221554251413643

    Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of TIE2 stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.
    Figure Legend Snippet: Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of TIE2 stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.

    Techniques Used: Staining, Membrane

    3D reconstructions of a kidney section. Figure shows 3D intensity reconstruction of the overall organization of two glomeruli (G) and adjacent parenchyma (P) across the depth (Z) of 60 μm; A: 3D reconstruction of the TIE2 stain, presenting image depth scale from 0 μm (blue) toward 60 μm (red). Different panels present the different plane views (XZ, XYZ, XY, YZ); B: 3D reconstruction presenting the TIE2 stain in the same planes as described above; C: 3D reconstruction presenting an overlay image of the WGA membrane stain (magenta) and the TIE2 stain (cyan) in the different planes described above.
    Figure Legend Snippet: 3D reconstructions of a kidney section. Figure shows 3D intensity reconstruction of the overall organization of two glomeruli (G) and adjacent parenchyma (P) across the depth (Z) of 60 μm; A: 3D reconstruction of the TIE2 stain, presenting image depth scale from 0 μm (blue) toward 60 μm (red). Different panels present the different plane views (XZ, XYZ, XY, YZ); B: 3D reconstruction presenting the TIE2 stain in the same planes as described above; C: 3D reconstruction presenting an overlay image of the WGA membrane stain (magenta) and the TIE2 stain (cyan) in the different planes described above.

    Techniques Used: Staining, Membrane

    Analysis strategy to determine the maximum signal intensity for the TIE2 and WGA stains on STED images. A, B: Freehand selection over the TIE2 signal (A) expressed on the top of a podocyte cell. The selection was copied via de ROI Manager for the WGA staining image (B); C, D: Clock Scan Combined selected area based on the ROI previously described, for signal intensity measurements on the TIE2 image (C), and WGA image (D). The Clock Scan protocol collects the pixel intensity in a radial manner, starting from the center of the ROI, clockwise, toward the edge of the ROI region (white arrows); E, F: Expression of the gray intensities for TIE2 (E) and WGA (F), measured with the Clock Scan Combined plugin; correction filters applied for the TIE2 channel: Brightness and Contrast—minimum displayed value 19; maximum displayed value 300. P = podocyte; scale bar 5 µm.
    Figure Legend Snippet: Analysis strategy to determine the maximum signal intensity for the TIE2 and WGA stains on STED images. A, B: Freehand selection over the TIE2 signal (A) expressed on the top of a podocyte cell. The selection was copied via de ROI Manager for the WGA staining image (B); C, D: Clock Scan Combined selected area based on the ROI previously described, for signal intensity measurements on the TIE2 image (C), and WGA image (D). The Clock Scan protocol collects the pixel intensity in a radial manner, starting from the center of the ROI, clockwise, toward the edge of the ROI region (white arrows); E, F: Expression of the gray intensities for TIE2 (E) and WGA (F), measured with the Clock Scan Combined plugin; correction filters applied for the TIE2 channel: Brightness and Contrast—minimum displayed value 19; maximum displayed value 300. P = podocyte; scale bar 5 µm.

    Techniques Used: Selection, Staining, Expressing

    Podocyte localization inside the glomerulum based on immunostaining with podocin. A: Podocytes (tip of white arrowheads) and slit diaphragm stained against podocin (green). B: HyD R detector normally used for TIE2 detection appears with minimal autofluorescence (red) as no target for TIE2 was used; C: Wheat germ agglutinin membrane stain (blue). Tip of the white arrows showcasing the podocyte cells from A. D: Composite of A, B, and C; scale bar 40 µm.
    Figure Legend Snippet: Podocyte localization inside the glomerulum based on immunostaining with podocin. A: Podocytes (tip of white arrowheads) and slit diaphragm stained against podocin (green). B: HyD R detector normally used for TIE2 detection appears with minimal autofluorescence (red) as no target for TIE2 was used; C: Wheat germ agglutinin membrane stain (blue). Tip of the white arrows showcasing the podocyte cells from A. D: Composite of A, B, and C; scale bar 40 µm.

    Techniques Used: Immunostaining, Staining, Membrane

    StarRED secondary antibody binds to both anti-TIE2 primary antibody and anti-podocin primary antibody—FLIM. A, B, C: Control experiment using the anti-podocin primary antibody [primary rabbit anti-podocin P0372 (Sigma-Aldrich)] and the Star RED secondary antibody [donkey anti-goat IgG StarRED 638 (Abberior), normally used to bind to the TIE2 primary antibody]. The StarRED secondary antibody in the FLIM channel (HyD R detector) shows immunofluorescent signal despite no primary antibody against TIE2 was used, indicating nonspecific binding of the Star RED secondary antibody to the anti-podocin antibody (A). The HyD X detector (donkey anti-rabbit A-21207 detection channel) shows no visible signal as expected (B). The phasor plot (C) shows the signal population corresponding to the nonspecific binding of the StarRED secondary antibody; D, E, F: Double immunofluorescence staining with primary antibodies against TIE2 and podocin shows two different lifetimes in the StarRED channel, marked in blue and green (yellow arrowheads in D), indicating contributions from both genuine TIE2 signal and nonspecific binding to the podocin antibody, and not a lifetime variation of the TIE2 binding to the slit diaphragm. The podocin FLIM channel shows only one lifetime marked in green (E). The phasor plot (F) shows the signal population corresponding to the StarRED (HyD R detector) and anti-podocin (HyD X detector) secondary antibodies; G, H, I: TIE2 population (white) and podocin population (magenta) (G) corresponding to the StarRED secondary antibody signal, gated on the FLIM image as observed on the phasor plot obtained from the HyD R detector (I). In the podocin FLIM channel (H) negligible signal corresponding to the StarRED marked population can be observed; scale bar 40 µm.
    Figure Legend Snippet: StarRED secondary antibody binds to both anti-TIE2 primary antibody and anti-podocin primary antibody—FLIM. A, B, C: Control experiment using the anti-podocin primary antibody [primary rabbit anti-podocin P0372 (Sigma-Aldrich)] and the Star RED secondary antibody [donkey anti-goat IgG StarRED 638 (Abberior), normally used to bind to the TIE2 primary antibody]. The StarRED secondary antibody in the FLIM channel (HyD R detector) shows immunofluorescent signal despite no primary antibody against TIE2 was used, indicating nonspecific binding of the Star RED secondary antibody to the anti-podocin antibody (A). The HyD X detector (donkey anti-rabbit A-21207 detection channel) shows no visible signal as expected (B). The phasor plot (C) shows the signal population corresponding to the nonspecific binding of the StarRED secondary antibody; D, E, F: Double immunofluorescence staining with primary antibodies against TIE2 and podocin shows two different lifetimes in the StarRED channel, marked in blue and green (yellow arrowheads in D), indicating contributions from both genuine TIE2 signal and nonspecific binding to the podocin antibody, and not a lifetime variation of the TIE2 binding to the slit diaphragm. The podocin FLIM channel shows only one lifetime marked in green (E). The phasor plot (F) shows the signal population corresponding to the StarRED (HyD R detector) and anti-podocin (HyD X detector) secondary antibodies; G, H, I: TIE2 population (white) and podocin population (magenta) (G) corresponding to the StarRED secondary antibody signal, gated on the FLIM image as observed on the phasor plot obtained from the HyD R detector (I). In the podocin FLIM channel (H) negligible signal corresponding to the StarRED marked population can be observed; scale bar 40 µm.

    Techniques Used: Control, Binding Assay, Double Immunofluorescence Staining

    TIE2 staining in the presence or absence of TIE2 recombinant protein. A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems). In the presence of the recombinant protein, the TIE2 specific signal inside the blood vasculature disappeared. Signal from the parenchyma remained visible, suggesting that the primary antibody binds nonspecific to the tissue and specific to the TIE2 target; E: Wheat Germ Agglutinin membrane stain of a kidney glomerulum (intensity image); F: Overlay of D and E (intensity image); scale bar 20 µm.
    Figure Legend Snippet: TIE2 staining in the presence or absence of TIE2 recombinant protein. A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems). In the presence of the recombinant protein, the TIE2 specific signal inside the blood vasculature disappeared. Signal from the parenchyma remained visible, suggesting that the primary antibody binds nonspecific to the tissue and specific to the TIE2 target; E: Wheat Germ Agglutinin membrane stain of a kidney glomerulum (intensity image); F: Overlay of D and E (intensity image); scale bar 20 µm.

    Techniques Used: Staining, Recombinant, Membrane

    Analysis strategy: determining autofluorescence, nonspecific binding of secondary antibody control and positive staining. A: Fast-FLIM image of tissue autofluorescence in the StarRED spectral channel. ROIs were traced over the red blood cells (RBC) (yellow arrowheads); B: Phasor plot representative for the autofluorescent ROI population determined in A; C, D: Gated autofluorescence of the red blood cells (yellow) (C) as marked on the overall phasor plot (D); E, G, I: Fast-FLIM image of secondary antibody nonspecific binding in the StarRED spectral channel. Different sets of ROIs were traced on the images, each set corresponding to one image (E, G, or I) (yellow arrowheads); F, H, J: Phasor plots representative for the secondary antibody nonspecific binding ROI populations, as traced on their corresponding image (E, G, or I); K, L: Gated nonspecific secondary antibody binding (red) and autofluorescence (yellow) (K) as marked on the overall phasor plot (L); M, O: Fast-FLIM image of TIE2 staining on the StarRED spectral channel. ROIs were traced based on morphological patterns and lifetime color coding over the blood vessel lumen (M) and podocyte cell body (O) (yellow arrowheads) excluding regions marked by the previous mentioned controls; N, P: Phasor plots representative for the vessel lumen TIE2 ROIs (N) and podocyte TIE2 ROIs (P); Q, R: Gated TIE2 signal population (white) (Q) as observed on the overall phasor plot in panel R; S: TIE2 signal population gated in white, nonspecific binding population gated in red and autofluorescence population gated in yellow on the Fast-FLIM image in the StarRED spectral channel as observed on the overall phasor plot in panel T; T: Phasor plot representing the overall fluorescence lifetime collected for the TIE2-stained samples. White circle delimits the TIE2 lifetime population, red circles determine the nonspecific binding of the secondary antibody populations and the yellow circle delimits the autofluorescent population; for visual aid and for the purpose of this representation only, white lines were traced, post-analysis, over the selected ROI regions; scale bar A, C 60 µm, E, G, I, K, M, O, Q, S 40 µm.
    Figure Legend Snippet: Analysis strategy: determining autofluorescence, nonspecific binding of secondary antibody control and positive staining. A: Fast-FLIM image of tissue autofluorescence in the StarRED spectral channel. ROIs were traced over the red blood cells (RBC) (yellow arrowheads); B: Phasor plot representative for the autofluorescent ROI population determined in A; C, D: Gated autofluorescence of the red blood cells (yellow) (C) as marked on the overall phasor plot (D); E, G, I: Fast-FLIM image of secondary antibody nonspecific binding in the StarRED spectral channel. Different sets of ROIs were traced on the images, each set corresponding to one image (E, G, or I) (yellow arrowheads); F, H, J: Phasor plots representative for the secondary antibody nonspecific binding ROI populations, as traced on their corresponding image (E, G, or I); K, L: Gated nonspecific secondary antibody binding (red) and autofluorescence (yellow) (K) as marked on the overall phasor plot (L); M, O: Fast-FLIM image of TIE2 staining on the StarRED spectral channel. ROIs were traced based on morphological patterns and lifetime color coding over the blood vessel lumen (M) and podocyte cell body (O) (yellow arrowheads) excluding regions marked by the previous mentioned controls; N, P: Phasor plots representative for the vessel lumen TIE2 ROIs (N) and podocyte TIE2 ROIs (P); Q, R: Gated TIE2 signal population (white) (Q) as observed on the overall phasor plot in panel R; S: TIE2 signal population gated in white, nonspecific binding population gated in red and autofluorescence population gated in yellow on the Fast-FLIM image in the StarRED spectral channel as observed on the overall phasor plot in panel T; T: Phasor plot representing the overall fluorescence lifetime collected for the TIE2-stained samples. White circle delimits the TIE2 lifetime population, red circles determine the nonspecific binding of the secondary antibody populations and the yellow circle delimits the autofluorescent population; for visual aid and for the purpose of this representation only, white lines were traced, post-analysis, over the selected ROI regions; scale bar A, C 60 µm, E, G, I, K, M, O, Q, S 40 µm.

    Techniques Used: Binding Assay, Control, Staining, Fluorescence

    Overlay of multiple phasor plot distribution coordinates of control and positive stained samples. A: Fluorescence lifetime distribution on the phasor plot of the autofluorescence RBC; B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody control C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody bound to anti-TIE2 primary antibody; D: Composite of panels A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime. To obtain the overlay images, all phasor plots analyzed within the experimental replicates were used.
    Figure Legend Snippet: Overlay of multiple phasor plot distribution coordinates of control and positive stained samples. A: Fluorescence lifetime distribution on the phasor plot of the autofluorescence RBC; B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody control C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody bound to anti-TIE2 primary antibody; D: Composite of panels A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime. To obtain the overlay images, all phasor plots analyzed within the experimental replicates were used.

    Techniques Used: Control, Staining, Fluorescence

    TIE2, autofluorescence, and nonspecific binding of the secondary antibody to the tissue group values comparison. Boxplot comparison of lifetime values obtained using phasor and fitting methods for three different groups: TIE2 signal (white), nonspecific binding of the secondary antibody control (red), and autofluorescence control (yellow).
    Figure Legend Snippet: TIE2, autofluorescence, and nonspecific binding of the secondary antibody to the tissue group values comparison. Boxplot comparison of lifetime values obtained using phasor and fitting methods for three different groups: TIE2 signal (white), nonspecific binding of the secondary antibody control (red), and autofluorescence control (yellow).

    Techniques Used: Binding Assay, Comparison, Control

    Fluorescence lifetime imaging microscopy reveals specific TIE2 signal in the glomerulus sections. L—vessel lumen; P—podocyte; arrowheads pinpoint at the TIE2 staining; A: TIE2 stain on podocyte and adjacent capillaries (intensity image); B: Wheat Germ Agglutinin membrane staining (intensity image); C: Overlay of A and B (intensity image); D, F: Fast-FLIM image of TIE2 staining (D) and TIE2 + control signals gated on the image (F); E, G: Zoom in from D and F on a podocyte (P) and blood vessel lumens (L) showcasing at the tip of the arrows the TIE2 stain in E and the Gated signal from the phasor plot corresponding to the TIE2 cluster in G. H: Phasor plot of the TIE2 lifetime signal cluster and the region of interest marked in white on the Fast-FLIM images (F and G). The phasor distribution also visualizes nonspecific binding of the secondary antibody (red) and tissue autofluorescence (yellow); scale bar: A, B, C, D, F 40 µm; E, G 5 µm.
    Figure Legend Snippet: Fluorescence lifetime imaging microscopy reveals specific TIE2 signal in the glomerulus sections. L—vessel lumen; P—podocyte; arrowheads pinpoint at the TIE2 staining; A: TIE2 stain on podocyte and adjacent capillaries (intensity image); B: Wheat Germ Agglutinin membrane staining (intensity image); C: Overlay of A and B (intensity image); D, F: Fast-FLIM image of TIE2 staining (D) and TIE2 + control signals gated on the image (F); E, G: Zoom in from D and F on a podocyte (P) and blood vessel lumens (L) showcasing at the tip of the arrows the TIE2 stain in E and the Gated signal from the phasor plot corresponding to the TIE2 cluster in G. H: Phasor plot of the TIE2 lifetime signal cluster and the region of interest marked in white on the Fast-FLIM images (F and G). The phasor distribution also visualizes nonspecific binding of the secondary antibody (red) and tissue autofluorescence (yellow); scale bar: A, B, C, D, F 40 µm; E, G 5 µm.

    Techniques Used: Fluorescence, Imaging, Microscopy, Staining, Membrane, Control, Binding Assay

    Fluorescence lifetime comparison between StarRED secondary antibody and StarRED bound to primary anti-TIE2 antibody in an in vitro droplet test. A: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml); B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (50 μg/ml); C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (100 μg/ml); D: Composite of panel A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime.
    Figure Legend Snippet: Fluorescence lifetime comparison between StarRED secondary antibody and StarRED bound to primary anti-TIE2 antibody in an in vitro droplet test. A: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml); B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (50 μg/ml); C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (100 μg/ml); D: Composite of panel A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime.

    Techniques Used: Fluorescence, Comparison, In Vitro

    TIE2 localization on glomerular podocytes revealed with STED microscopy. L = vessel lumen; P = podocyte; A, B: Podocyte and adjacent capillary lumen stained against TIE2 (A) and WGA (B); C: Composite of A and B; D, E: TIE2 (D) and WGA (E) staining converted to grayscale, with indicated region for Clock Scan analysis profile (yellow ROI); F: Signal intensity position within the selected area in D and E, measured with the Clock Scan Combined plugin, representing TIE2 (cyan) and WGA (magenta). G: Box plots displaying the position of the maximum pixel intensity values for the TIE2 channel (cyan) and the WGA channel (magenta) from all the measured images; scale bar 5 µm.
    Figure Legend Snippet: TIE2 localization on glomerular podocytes revealed with STED microscopy. L = vessel lumen; P = podocyte; A, B: Podocyte and adjacent capillary lumen stained against TIE2 (A) and WGA (B); C: Composite of A and B; D, E: TIE2 (D) and WGA (E) staining converted to grayscale, with indicated region for Clock Scan analysis profile (yellow ROI); F: Signal intensity position within the selected area in D and E, measured with the Clock Scan Combined plugin, representing TIE2 (cyan) and WGA (magenta). G: Box plots displaying the position of the maximum pixel intensity values for the TIE2 channel (cyan) and the WGA channel (magenta) from all the measured images; scale bar 5 µm.

    Techniques Used: Microscopy, Staining

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Platelet granule secretion continuously prevents intratumor hemorrhage
    Article Snippet: VEGF levels in platelet-poor plasma (PPP), serum and tumor were assayed using an ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. .. Angiopoietin-1 levels were quantified by ELISA using glutaraldehyde-immobilized recombinant mouse Tie2-Fc chimera (R&D Systems) for capture and a goat polyclonal to angiopoietin-1 (Santa Cruz Biotechnology, Santa Cruz, CA) for detection. .. In addition, PPP and serum samples from control and platelet-depleted mice were analyzed by SDS-PAGE (10 %, reducing conditions) and Western blotting with a goat polyclonal antibody to angiopoietin-1 (Santa Cruz Biotechnology).

    Article Title: Platelet Granule Secretion Continuously Prevents Intratumor Hemorrhage
    Article Snippet: VEGF levels in platelet-poor plasma (PPP), serum, and tumor were assayed using an ELISA kit (R&D Systems) according to the manufacturer’s instructions. .. Angiopoietin-1 levels were quantified by ELISA using glutaraldehydeimmobilized recombinant mouse Tie2-Fc chimera (R&D Systems) for capture and a goat polyclonal to angiopoietin-1 (Santa Cruz Biotechnology) for detection. ..

    Recombinant:

    Article Title: Platelet granule secretion continuously prevents intratumor hemorrhage
    Article Snippet: VEGF levels in platelet-poor plasma (PPP), serum and tumor were assayed using an ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. .. Angiopoietin-1 levels were quantified by ELISA using glutaraldehyde-immobilized recombinant mouse Tie2-Fc chimera (R&D Systems) for capture and a goat polyclonal to angiopoietin-1 (Santa Cruz Biotechnology, Santa Cruz, CA) for detection. .. In addition, PPP and serum samples from control and platelet-depleted mice were analyzed by SDS-PAGE (10 %, reducing conditions) and Western blotting with a goat polyclonal antibody to angiopoietin-1 (Santa Cruz Biotechnology).

    Article Title: Transcriptional and posttranscriptional regulation of angiopoietin-2 expression mediated by IGF and PDGF in vascular smooth muscle cells.
    Article Snippet: Phelps, Eric D., Dawn L. Updike, Elizabeth C. Bullen, Paula Grammas, and Eric W. Howard.. Transcriptional and posttranscriptional regulation of angiopoietin-2 expression mediated by IGF and PDGF in vascular smooth muscle cells.. Am J Physiol Cell Physiol 290: C352–C361, 2006.

    Article Title: Platelet Granule Secretion Continuously Prevents Intratumor Hemorrhage
    Article Snippet: VEGF levels in platelet-poor plasma (PPP), serum, and tumor were assayed using an ELISA kit (R&D Systems) according to the manufacturer’s instructions. .. Angiopoietin-1 levels were quantified by ELISA using glutaraldehydeimmobilized recombinant mouse Tie2-Fc chimera (R&D Systems) for capture and a goat polyclonal to angiopoietin-1 (Santa Cruz Biotechnology) for detection. ..



    Similar Products

    tie2 recombinant protein  (R&D Systems)
    94
    R&D Systems tie2 recombinant protein
    Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of <t>TIE2</t> stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.
    Tie2 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/pmc12823351-218-39-51
    Average 94 stars, based on 1 article reviews
    tie2 recombinant protein - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    mouse tie2 fc chimeric protein  (R&D Systems)
    94
    R&D Systems mouse tie2 fc chimeric protein
    Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of <t>TIE2</t> stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.
    Mouse Tie2 Fc Chimeric Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/pmc08263585-176-4-8
    Average 94 stars, based on 1 article reviews
    mouse tie2 fc chimeric protein - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    fc tie2  (R&D Systems)
    91
    R&D Systems fc tie2
    Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + <t>/Tie2</t> + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.
    Fc Tie2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/pmc07760857-224-4-10
    Average 91 stars, based on 1 article reviews
    fc tie2 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    recombinant mouse tie2 fc chimeric protein  (R&D Systems Hematology)
    94
    R&D Systems Hematology recombinant mouse tie2 fc chimeric protein
    Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + <t>/Tie2</t> + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.
    Recombinant Mouse Tie2 Fc Chimeric Protein, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/us09902782-558-28-33
    Average 94 stars, based on 1 article reviews
    recombinant mouse tie2 fc chimeric protein - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    mouse tie2 fc chimera  (R&D Systems)
    93
    R&D Systems mouse tie2 fc chimera
    Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + <t>/Tie2</t> + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.
    Mouse Tie2 Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Human+Tie-2+Fc+Chimera+Protein%2C+CF/pmc04459817__emmm0007___0770___sd1-72-3-10
    Average 93 stars, based on 1 article reviews
    mouse tie2 fc chimera - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mcao wt tie2 fc  (R&D Systems)
    94
    R&D Systems mcao wt tie2 fc
    Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + <t>/Tie2</t> + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.
    Mcao Wt Tie2 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tie2+fc+chimera/Recombinant+Mouse+Tie-2+Fc+Chimera+Protein%2C+CF/pmc03096677-98-18-26
    Average 94 stars, based on 1 article reviews
    mcao wt tie2 fc - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of TIE2 stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Overview of analyzed kidney tissue using mosaic scan. A: Mosaic scan of TIE2 stain on kidney tissue (intensity image); B: Mosaic scan of Wheat Germ Agglutinin membrane staining on kidney tissue (intensity image); C: Overlay of A and B (intensity image); scale bar 100 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Staining, Membrane

    3D reconstructions of a kidney section. Figure shows 3D intensity reconstruction of the overall organization of two glomeruli (G) and adjacent parenchyma (P) across the depth (Z) of 60 μm; A: 3D reconstruction of the TIE2 stain, presenting image depth scale from 0 μm (blue) toward 60 μm (red). Different panels present the different plane views (XZ, XYZ, XY, YZ); B: 3D reconstruction presenting the TIE2 stain in the same planes as described above; C: 3D reconstruction presenting an overlay image of the WGA membrane stain (magenta) and the TIE2 stain (cyan) in the different planes described above.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: 3D reconstructions of a kidney section. Figure shows 3D intensity reconstruction of the overall organization of two glomeruli (G) and adjacent parenchyma (P) across the depth (Z) of 60 μm; A: 3D reconstruction of the TIE2 stain, presenting image depth scale from 0 μm (blue) toward 60 μm (red). Different panels present the different plane views (XZ, XYZ, XY, YZ); B: 3D reconstruction presenting the TIE2 stain in the same planes as described above; C: 3D reconstruction presenting an overlay image of the WGA membrane stain (magenta) and the TIE2 stain (cyan) in the different planes described above.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Staining, Membrane

    Analysis strategy to determine the maximum signal intensity for the TIE2 and WGA stains on STED images. A, B: Freehand selection over the TIE2 signal (A) expressed on the top of a podocyte cell. The selection was copied via de ROI Manager for the WGA staining image (B); C, D: Clock Scan Combined selected area based on the ROI previously described, for signal intensity measurements on the TIE2 image (C), and WGA image (D). The Clock Scan protocol collects the pixel intensity in a radial manner, starting from the center of the ROI, clockwise, toward the edge of the ROI region (white arrows); E, F: Expression of the gray intensities for TIE2 (E) and WGA (F), measured with the Clock Scan Combined plugin; correction filters applied for the TIE2 channel: Brightness and Contrast—minimum displayed value 19; maximum displayed value 300. P = podocyte; scale bar 5 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Analysis strategy to determine the maximum signal intensity for the TIE2 and WGA stains on STED images. A, B: Freehand selection over the TIE2 signal (A) expressed on the top of a podocyte cell. The selection was copied via de ROI Manager for the WGA staining image (B); C, D: Clock Scan Combined selected area based on the ROI previously described, for signal intensity measurements on the TIE2 image (C), and WGA image (D). The Clock Scan protocol collects the pixel intensity in a radial manner, starting from the center of the ROI, clockwise, toward the edge of the ROI region (white arrows); E, F: Expression of the gray intensities for TIE2 (E) and WGA (F), measured with the Clock Scan Combined plugin; correction filters applied for the TIE2 channel: Brightness and Contrast—minimum displayed value 19; maximum displayed value 300. P = podocyte; scale bar 5 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Selection, Staining, Expressing

    Podocyte localization inside the glomerulum based on immunostaining with podocin. A: Podocytes (tip of white arrowheads) and slit diaphragm stained against podocin (green). B: HyD R detector normally used for TIE2 detection appears with minimal autofluorescence (red) as no target for TIE2 was used; C: Wheat germ agglutinin membrane stain (blue). Tip of the white arrows showcasing the podocyte cells from A. D: Composite of A, B, and C; scale bar 40 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Podocyte localization inside the glomerulum based on immunostaining with podocin. A: Podocytes (tip of white arrowheads) and slit diaphragm stained against podocin (green). B: HyD R detector normally used for TIE2 detection appears with minimal autofluorescence (red) as no target for TIE2 was used; C: Wheat germ agglutinin membrane stain (blue). Tip of the white arrows showcasing the podocyte cells from A. D: Composite of A, B, and C; scale bar 40 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Immunostaining, Staining, Membrane

    StarRED secondary antibody binds to both anti-TIE2 primary antibody and anti-podocin primary antibody—FLIM. A, B, C: Control experiment using the anti-podocin primary antibody [primary rabbit anti-podocin P0372 (Sigma-Aldrich)] and the Star RED secondary antibody [donkey anti-goat IgG StarRED 638 (Abberior), normally used to bind to the TIE2 primary antibody]. The StarRED secondary antibody in the FLIM channel (HyD R detector) shows immunofluorescent signal despite no primary antibody against TIE2 was used, indicating nonspecific binding of the Star RED secondary antibody to the anti-podocin antibody (A). The HyD X detector (donkey anti-rabbit A-21207 detection channel) shows no visible signal as expected (B). The phasor plot (C) shows the signal population corresponding to the nonspecific binding of the StarRED secondary antibody; D, E, F: Double immunofluorescence staining with primary antibodies against TIE2 and podocin shows two different lifetimes in the StarRED channel, marked in blue and green (yellow arrowheads in D), indicating contributions from both genuine TIE2 signal and nonspecific binding to the podocin antibody, and not a lifetime variation of the TIE2 binding to the slit diaphragm. The podocin FLIM channel shows only one lifetime marked in green (E). The phasor plot (F) shows the signal population corresponding to the StarRED (HyD R detector) and anti-podocin (HyD X detector) secondary antibodies; G, H, I: TIE2 population (white) and podocin population (magenta) (G) corresponding to the StarRED secondary antibody signal, gated on the FLIM image as observed on the phasor plot obtained from the HyD R detector (I). In the podocin FLIM channel (H) negligible signal corresponding to the StarRED marked population can be observed; scale bar 40 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: StarRED secondary antibody binds to both anti-TIE2 primary antibody and anti-podocin primary antibody—FLIM. A, B, C: Control experiment using the anti-podocin primary antibody [primary rabbit anti-podocin P0372 (Sigma-Aldrich)] and the Star RED secondary antibody [donkey anti-goat IgG StarRED 638 (Abberior), normally used to bind to the TIE2 primary antibody]. The StarRED secondary antibody in the FLIM channel (HyD R detector) shows immunofluorescent signal despite no primary antibody against TIE2 was used, indicating nonspecific binding of the Star RED secondary antibody to the anti-podocin antibody (A). The HyD X detector (donkey anti-rabbit A-21207 detection channel) shows no visible signal as expected (B). The phasor plot (C) shows the signal population corresponding to the nonspecific binding of the StarRED secondary antibody; D, E, F: Double immunofluorescence staining with primary antibodies against TIE2 and podocin shows two different lifetimes in the StarRED channel, marked in blue and green (yellow arrowheads in D), indicating contributions from both genuine TIE2 signal and nonspecific binding to the podocin antibody, and not a lifetime variation of the TIE2 binding to the slit diaphragm. The podocin FLIM channel shows only one lifetime marked in green (E). The phasor plot (F) shows the signal population corresponding to the StarRED (HyD R detector) and anti-podocin (HyD X detector) secondary antibodies; G, H, I: TIE2 population (white) and podocin population (magenta) (G) corresponding to the StarRED secondary antibody signal, gated on the FLIM image as observed on the phasor plot obtained from the HyD R detector (I). In the podocin FLIM channel (H) negligible signal corresponding to the StarRED marked population can be observed; scale bar 40 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Control, Binding Assay, Double Immunofluorescence Staining

    TIE2 staining in the presence or absence of TIE2 recombinant protein. A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems). In the presence of the recombinant protein, the TIE2 specific signal inside the blood vasculature disappeared. Signal from the parenchyma remained visible, suggesting that the primary antibody binds nonspecific to the tissue and specific to the TIE2 target; E: Wheat Germ Agglutinin membrane stain of a kidney glomerulum (intensity image); F: Overlay of D and E (intensity image); scale bar 20 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: TIE2 staining in the presence or absence of TIE2 recombinant protein. A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems). In the presence of the recombinant protein, the TIE2 specific signal inside the blood vasculature disappeared. Signal from the parenchyma remained visible, suggesting that the primary antibody binds nonspecific to the tissue and specific to the TIE2 target; E: Wheat Germ Agglutinin membrane stain of a kidney glomerulum (intensity image); F: Overlay of D and E (intensity image); scale bar 20 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Staining, Recombinant, Membrane

    Analysis strategy: determining autofluorescence, nonspecific binding of secondary antibody control and positive staining. A: Fast-FLIM image of tissue autofluorescence in the StarRED spectral channel. ROIs were traced over the red blood cells (RBC) (yellow arrowheads); B: Phasor plot representative for the autofluorescent ROI population determined in A; C, D: Gated autofluorescence of the red blood cells (yellow) (C) as marked on the overall phasor plot (D); E, G, I: Fast-FLIM image of secondary antibody nonspecific binding in the StarRED spectral channel. Different sets of ROIs were traced on the images, each set corresponding to one image (E, G, or I) (yellow arrowheads); F, H, J: Phasor plots representative for the secondary antibody nonspecific binding ROI populations, as traced on their corresponding image (E, G, or I); K, L: Gated nonspecific secondary antibody binding (red) and autofluorescence (yellow) (K) as marked on the overall phasor plot (L); M, O: Fast-FLIM image of TIE2 staining on the StarRED spectral channel. ROIs were traced based on morphological patterns and lifetime color coding over the blood vessel lumen (M) and podocyte cell body (O) (yellow arrowheads) excluding regions marked by the previous mentioned controls; N, P: Phasor plots representative for the vessel lumen TIE2 ROIs (N) and podocyte TIE2 ROIs (P); Q, R: Gated TIE2 signal population (white) (Q) as observed on the overall phasor plot in panel R; S: TIE2 signal population gated in white, nonspecific binding population gated in red and autofluorescence population gated in yellow on the Fast-FLIM image in the StarRED spectral channel as observed on the overall phasor plot in panel T; T: Phasor plot representing the overall fluorescence lifetime collected for the TIE2-stained samples. White circle delimits the TIE2 lifetime population, red circles determine the nonspecific binding of the secondary antibody populations and the yellow circle delimits the autofluorescent population; for visual aid and for the purpose of this representation only, white lines were traced, post-analysis, over the selected ROI regions; scale bar A, C 60 µm, E, G, I, K, M, O, Q, S 40 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Analysis strategy: determining autofluorescence, nonspecific binding of secondary antibody control and positive staining. A: Fast-FLIM image of tissue autofluorescence in the StarRED spectral channel. ROIs were traced over the red blood cells (RBC) (yellow arrowheads); B: Phasor plot representative for the autofluorescent ROI population determined in A; C, D: Gated autofluorescence of the red blood cells (yellow) (C) as marked on the overall phasor plot (D); E, G, I: Fast-FLIM image of secondary antibody nonspecific binding in the StarRED spectral channel. Different sets of ROIs were traced on the images, each set corresponding to one image (E, G, or I) (yellow arrowheads); F, H, J: Phasor plots representative for the secondary antibody nonspecific binding ROI populations, as traced on their corresponding image (E, G, or I); K, L: Gated nonspecific secondary antibody binding (red) and autofluorescence (yellow) (K) as marked on the overall phasor plot (L); M, O: Fast-FLIM image of TIE2 staining on the StarRED spectral channel. ROIs were traced based on morphological patterns and lifetime color coding over the blood vessel lumen (M) and podocyte cell body (O) (yellow arrowheads) excluding regions marked by the previous mentioned controls; N, P: Phasor plots representative for the vessel lumen TIE2 ROIs (N) and podocyte TIE2 ROIs (P); Q, R: Gated TIE2 signal population (white) (Q) as observed on the overall phasor plot in panel R; S: TIE2 signal population gated in white, nonspecific binding population gated in red and autofluorescence population gated in yellow on the Fast-FLIM image in the StarRED spectral channel as observed on the overall phasor plot in panel T; T: Phasor plot representing the overall fluorescence lifetime collected for the TIE2-stained samples. White circle delimits the TIE2 lifetime population, red circles determine the nonspecific binding of the secondary antibody populations and the yellow circle delimits the autofluorescent population; for visual aid and for the purpose of this representation only, white lines were traced, post-analysis, over the selected ROI regions; scale bar A, C 60 µm, E, G, I, K, M, O, Q, S 40 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Binding Assay, Control, Staining, Fluorescence

    Overlay of multiple phasor plot distribution coordinates of control and positive stained samples. A: Fluorescence lifetime distribution on the phasor plot of the autofluorescence RBC; B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody control C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody bound to anti-TIE2 primary antibody; D: Composite of panels A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime. To obtain the overlay images, all phasor plots analyzed within the experimental replicates were used.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Overlay of multiple phasor plot distribution coordinates of control and positive stained samples. A: Fluorescence lifetime distribution on the phasor plot of the autofluorescence RBC; B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody control C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody bound to anti-TIE2 primary antibody; D: Composite of panels A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime. To obtain the overlay images, all phasor plots analyzed within the experimental replicates were used.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Control, Staining, Fluorescence

    TIE2, autofluorescence, and nonspecific binding of the secondary antibody to the tissue group values comparison. Boxplot comparison of lifetime values obtained using phasor and fitting methods for three different groups: TIE2 signal (white), nonspecific binding of the secondary antibody control (red), and autofluorescence control (yellow).

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: TIE2, autofluorescence, and nonspecific binding of the secondary antibody to the tissue group values comparison. Boxplot comparison of lifetime values obtained using phasor and fitting methods for three different groups: TIE2 signal (white), nonspecific binding of the secondary antibody control (red), and autofluorescence control (yellow).

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Binding Assay, Comparison, Control

    Fluorescence lifetime imaging microscopy reveals specific TIE2 signal in the glomerulus sections. L—vessel lumen; P—podocyte; arrowheads pinpoint at the TIE2 staining; A: TIE2 stain on podocyte and adjacent capillaries (intensity image); B: Wheat Germ Agglutinin membrane staining (intensity image); C: Overlay of A and B (intensity image); D, F: Fast-FLIM image of TIE2 staining (D) and TIE2 + control signals gated on the image (F); E, G: Zoom in from D and F on a podocyte (P) and blood vessel lumens (L) showcasing at the tip of the arrows the TIE2 stain in E and the Gated signal from the phasor plot corresponding to the TIE2 cluster in G. H: Phasor plot of the TIE2 lifetime signal cluster and the region of interest marked in white on the Fast-FLIM images (F and G). The phasor distribution also visualizes nonspecific binding of the secondary antibody (red) and tissue autofluorescence (yellow); scale bar: A, B, C, D, F 40 µm; E, G 5 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Fluorescence lifetime imaging microscopy reveals specific TIE2 signal in the glomerulus sections. L—vessel lumen; P—podocyte; arrowheads pinpoint at the TIE2 staining; A: TIE2 stain on podocyte and adjacent capillaries (intensity image); B: Wheat Germ Agglutinin membrane staining (intensity image); C: Overlay of A and B (intensity image); D, F: Fast-FLIM image of TIE2 staining (D) and TIE2 + control signals gated on the image (F); E, G: Zoom in from D and F on a podocyte (P) and blood vessel lumens (L) showcasing at the tip of the arrows the TIE2 stain in E and the Gated signal from the phasor plot corresponding to the TIE2 cluster in G. H: Phasor plot of the TIE2 lifetime signal cluster and the region of interest marked in white on the Fast-FLIM images (F and G). The phasor distribution also visualizes nonspecific binding of the secondary antibody (red) and tissue autofluorescence (yellow); scale bar: A, B, C, D, F 40 µm; E, G 5 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Fluorescence, Imaging, Microscopy, Staining, Membrane, Control, Binding Assay

    Fluorescence lifetime comparison between StarRED secondary antibody and StarRED bound to primary anti-TIE2 antibody in an in vitro droplet test. A: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml); B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (50 μg/ml); C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (100 μg/ml); D: Composite of panel A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: Fluorescence lifetime comparison between StarRED secondary antibody and StarRED bound to primary anti-TIE2 antibody in an in vitro droplet test. A: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml); B: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (50 μg/ml); C: Fluorescence lifetime distribution on the phasor plot of the StarRED secondary antibody (400 μg/ml) bound to anti-TIE2 primary antibody (100 μg/ml); D: Composite of panel A, B, and C. It can be observed from the image that the addition of the primary antibody shifts the overall measured fluorescence lifetime.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Fluorescence, Comparison, In Vitro

    TIE2 localization on glomerular podocytes revealed with STED microscopy. L = vessel lumen; P = podocyte; A, B: Podocyte and adjacent capillary lumen stained against TIE2 (A) and WGA (B); C: Composite of A and B; D, E: TIE2 (D) and WGA (E) staining converted to grayscale, with indicated region for Clock Scan analysis profile (yellow ROI); F: Signal intensity position within the selected area in D and E, measured with the Clock Scan Combined plugin, representing TIE2 (cyan) and WGA (magenta). G: Box plots displaying the position of the maximum pixel intensity values for the TIE2 channel (cyan) and the WGA channel (magenta) from all the measured images; scale bar 5 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes

    doi: 10.1369/00221554251413643

    Figure Lengend Snippet: TIE2 localization on glomerular podocytes revealed with STED microscopy. L = vessel lumen; P = podocyte; A, B: Podocyte and adjacent capillary lumen stained against TIE2 (A) and WGA (B); C: Composite of A and B; D, E: TIE2 (D) and WGA (E) staining converted to grayscale, with indicated region for Clock Scan analysis profile (yellow ROI); F: Signal intensity position within the selected area in D and E, measured with the Clock Scan Combined plugin, representing TIE2 (cyan) and WGA (magenta). G: Box plots displaying the position of the maximum pixel intensity values for the TIE2 channel (cyan) and the WGA channel (magenta) from all the measured images; scale bar 5 µm.

    Article Snippet: A: TIE2 staining of a kidney glomerulum (intensity image); B: Wheat Germ Agglutinin membrane staining on a kidney glomerulum (intensity image); C: Overlay of A and B; D: TIE2 staining of a kidney glomerulum in the presence of a TIE2 recombinant protein (Recombinant Mouse Tie-2 Fc Chimera Protein, catalog number 762-T2, R&D Systems).

    Techniques: Microscopy, Staining

    Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + /Tie2 + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.

    Journal: Cancers

    Article Title: Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study

    doi: 10.3390/cancers12123585

    Figure Lengend Snippet: Ang2 overexpression in glioblastoma cells combined with radiochemotherapy favored inflammation in glioblastoma. ( A ) Representative images of CD68 immunostaining at D14 and quantification of CD68 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( B ) Representative images of CD68 + /Tie2 + cells in GL261-Ang2 tumor-bearing mice. Scale bar = 100 µm and 50 µm for magnification. ( C ) Representative images of CD4 immunostaining at D14 and quantification of CD4 + area. Scale bar = 100 µm. n = 3 animals for each group. Mean ± SD # p < 0.05 vs. the respective untreated group, ANOVA followed by Tukey’s test. ( D ) Representative images of CD8 immunostaining at D14 and quantification of CD8 + area. Scale bar = 100 µm. n = 3 animals for each group. ( E ) Representative T2w images of the GL261-Ang2 tumor at D42 and D100 after cell implantation. Dashed lines delimit the residual tumor area and correspond to the representative images obtained from the immunohistological study to detect immune cells (CD68 + , CD4 + , and CD8 + ). Scale bar = 100 µm.

    Article Snippet: To evaluate Ang2 contribution, Fc Tie2 at 8 μg/mL (762-T2, R&D Systems, Lille, France) was added 30 min before adding the transwell into the plate.

    Techniques: Over Expression, Immunostaining

    Ang2 induced macrophage migration in vitro. ( A ) Representative images and quantification of migrated RAW 264.7 cells in response to recombinant Ang2. Scale bar = 50 µm; mean ± SD, * p < 0.05 vs. 0 ng/mL Ang2, # p < 0.05 vs. Fc Tie2 condition, two-way ANOVA followed by Tukey’s test. ( B ) Representative images and quantification of migrating RAW 264.7 cells in response to conditioned medium from tumor cells. Scale bar = 50 µm; Mean ± SD, n = 4; * p < 0.05 vs. GL261-wt group, # p < 0.05 vs. Fc Tie2 condition, two-way ANOVA followed by Tukey’s test.

    Journal: Cancers

    Article Title: Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study

    doi: 10.3390/cancers12123585

    Figure Lengend Snippet: Ang2 induced macrophage migration in vitro. ( A ) Representative images and quantification of migrated RAW 264.7 cells in response to recombinant Ang2. Scale bar = 50 µm; mean ± SD, * p < 0.05 vs. 0 ng/mL Ang2, # p < 0.05 vs. Fc Tie2 condition, two-way ANOVA followed by Tukey’s test. ( B ) Representative images and quantification of migrating RAW 264.7 cells in response to conditioned medium from tumor cells. Scale bar = 50 µm; Mean ± SD, n = 4; * p < 0.05 vs. GL261-wt group, # p < 0.05 vs. Fc Tie2 condition, two-way ANOVA followed by Tukey’s test.

    Article Snippet: To evaluate Ang2 contribution, Fc Tie2 at 8 μg/mL (762-T2, R&D Systems, Lille, France) was added 30 min before adding the transwell into the plate.

    Techniques: Migration, In Vitro, Recombinant